rabbit α-s100b Search Results


rabbit α-s100b  (GeneTex)
90
GeneTex rabbit α-s100b
Primer sequences and general conditions used to perform real-time qPCR
Rabbit α S100b, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+%CE%B1-s100b/s100+calcium+binding+protein++s100++a8+rabbit+polyclonal+antibody/pmc06307226-14-0-3
Average 90 stars, based on 1 article reviews
rabbit α-s100b - by Bioz Stars, 2026-10
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Primer sequences and general conditions used to perform real-time qPCR

Journal: Molecular Autism

Article Title: Neuroglia in the autistic brain: evidence from a preclinical model

doi: 10.1186/s13229-018-0254-0

Figure Lengend Snippet: Primer sequences and general conditions used to perform real-time qPCR

Article Snippet: Rabbit α-S100B , Genetex , 1:1000 , HRP conjugated goat anti-rabbit IgG 1:10000 , Jackson ImmunoResearch.

Techniques:

Experimental conditions used to perform western blot experiments

Journal: Molecular Autism

Article Title: Neuroglia in the autistic brain: evidence from a preclinical model

doi: 10.1186/s13229-018-0254-0

Figure Lengend Snippet: Experimental conditions used to perform western blot experiments

Article Snippet: Rabbit α-S100B , Genetex , 1:1000 , HRP conjugated goat anti-rabbit IgG 1:10000 , Jackson ImmunoResearch.

Techniques: Western Blot

Effect of prenatal VPA exposure on astrocytes in infancy. Analysis of the neurotrophin S100B and the GFAP in the PfC, Cb, and HPC of healthy- (open bars, Veh) and autistic-like (black bars, VPA) infant rats (PND 13). Representation of the relative concentration of S100B ( a ) and GFAP ( c ) in VPA animals compared to control (Veh), normalized to both TBP and HPRT (ΔΔCq; N = 3, in triplicate). Representative western blots for S100B ( b ) and GFAP ( d ) proteins, and densitometric analyses are normalized to β-actin used as loading control. Results are expressed as percentage of control (Veh) ( N = 3, in triplicate). Representative fluorescence micrographs of GFAP (green) staining in the PfCGL and MLof Cb, and CA1, CA2, CA3, and the hilus of the DG of the HPC. Nuclei were stained with Hoechst (blue) ( e ). The images have been analyzed by counting the number of GFAP-positive cells in 2.4 × 10 5 μm 3 (scale bar 50 μm; N = 3, 4 times) ( f ). All data are presented as means ± SEM. Statistical analysis was performed by t test (* p < 0.05; *** p < 0.001 vs Veh group)

Journal: Molecular Autism

Article Title: Neuroglia in the autistic brain: evidence from a preclinical model

doi: 10.1186/s13229-018-0254-0

Figure Lengend Snippet: Effect of prenatal VPA exposure on astrocytes in infancy. Analysis of the neurotrophin S100B and the GFAP in the PfC, Cb, and HPC of healthy- (open bars, Veh) and autistic-like (black bars, VPA) infant rats (PND 13). Representation of the relative concentration of S100B ( a ) and GFAP ( c ) in VPA animals compared to control (Veh), normalized to both TBP and HPRT (ΔΔCq; N = 3, in triplicate). Representative western blots for S100B ( b ) and GFAP ( d ) proteins, and densitometric analyses are normalized to β-actin used as loading control. Results are expressed as percentage of control (Veh) ( N = 3, in triplicate). Representative fluorescence micrographs of GFAP (green) staining in the PfCGL and MLof Cb, and CA1, CA2, CA3, and the hilus of the DG of the HPC. Nuclei were stained with Hoechst (blue) ( e ). The images have been analyzed by counting the number of GFAP-positive cells in 2.4 × 10 5 μm 3 (scale bar 50 μm; N = 3, 4 times) ( f ). All data are presented as means ± SEM. Statistical analysis was performed by t test (* p < 0.05; *** p < 0.001 vs Veh group)

Article Snippet: Rabbit α-S100B , Genetex , 1:1000 , HRP conjugated goat anti-rabbit IgG 1:10000 , Jackson ImmunoResearch.

Techniques: Concentration Assay, Control, Western Blot, Fluorescence, Staining

Effect of the prenatal VPA exposure on astrocytes in adolescence. Evaluation of the neurotrophin S100B and the cytoskeletal GFAP in the PfC, Cb, and HPC of healthy- (open bars, Veh) and autistic-like (black bars, VPA) adolescent rats (PND 35). Representation of the relative concentration of S100B ( a ) and GFAP ( c ) in VPA animals compared to control (Veh), normalized to both TBP and HPRT (ΔΔCq; N = 3, in triplicate). Representative western blots for S100B ( b ) and GFAP ( d ) proteins, and densitometric analyses normalized to β-actin used as loading control. Results are expressed as percentage of control (Veh) ( N = 3, in triplicate). Representative fluorescence micrographs of GFAP (green) staining in the PfC, GL and ML of Cb, and stratum radiatum of CA1, CA2, CA3, and the hilus of the DG of the HPC. Nuclei were stained with Hoechst (blue) ( e ). The images have been analyzed by counting the number of GFAP-positive cells in 2.4 × 10 5 μm 3 (scale bar 50 μm; N = 3, 4 times) ( f ). All data are presented as means ± SEM. Statistical analysis was performed by t test (* p < 0.05; ** p < 0.01; *** p < 0.001 vs Veh group)

Journal: Molecular Autism

Article Title: Neuroglia in the autistic brain: evidence from a preclinical model

doi: 10.1186/s13229-018-0254-0

Figure Lengend Snippet: Effect of the prenatal VPA exposure on astrocytes in adolescence. Evaluation of the neurotrophin S100B and the cytoskeletal GFAP in the PfC, Cb, and HPC of healthy- (open bars, Veh) and autistic-like (black bars, VPA) adolescent rats (PND 35). Representation of the relative concentration of S100B ( a ) and GFAP ( c ) in VPA animals compared to control (Veh), normalized to both TBP and HPRT (ΔΔCq; N = 3, in triplicate). Representative western blots for S100B ( b ) and GFAP ( d ) proteins, and densitometric analyses normalized to β-actin used as loading control. Results are expressed as percentage of control (Veh) ( N = 3, in triplicate). Representative fluorescence micrographs of GFAP (green) staining in the PfC, GL and ML of Cb, and stratum radiatum of CA1, CA2, CA3, and the hilus of the DG of the HPC. Nuclei were stained with Hoechst (blue) ( e ). The images have been analyzed by counting the number of GFAP-positive cells in 2.4 × 10 5 μm 3 (scale bar 50 μm; N = 3, 4 times) ( f ). All data are presented as means ± SEM. Statistical analysis was performed by t test (* p < 0.05; ** p < 0.01; *** p < 0.001 vs Veh group)

Article Snippet: Rabbit α-S100B , Genetex , 1:1000 , HRP conjugated goat anti-rabbit IgG 1:10000 , Jackson ImmunoResearch.

Techniques: Concentration Assay, Control, Western Blot, Fluorescence, Staining

Effect of the prenatal VPA exposure on astrocytes in adulthood. Evaluation of the neurotrophin S100B and the cytoskeletal GFAP in the PfC, Cb, and HPC of healthy- (open bars, Veh) and autistic-like (black bars, VPA) adult rats (PND 90). Representation of the relative concentration of S100B ( a ) and GFAP ( c ) in VPA animals compared to control (Veh), normalized to both TBP and HPRT (ΔΔCq; N = 3, in triplicate). Representative western blots for S100B ( b ) and GFAP ( d ) proteins, and densitometric analyses normalized to β-actin used as loading control. Results are expressed as percentage of control (Veh) ( N = 3, in triplicate). Representative fluorescence micrographs of GFAP (green) staining in the PfC, GL and ML of Cb, and stratum radiatum of CA1, CA2, CA3, and the hilus of the DG of the HPC. Nuclei were stained with Hoechst (blue) ( e ). The images have been analyzed by counting the number of GFAP-positive cells in 2.4 × 10 5 μm 3 (scale bar 50 μm; N = 3, 4 times) ( f ). All data are presented as means ± SEM. Statistical analysis was performed by t test (* p < 0.05; *** p < 0.001 vs Veh group)

Journal: Molecular Autism

Article Title: Neuroglia in the autistic brain: evidence from a preclinical model

doi: 10.1186/s13229-018-0254-0

Figure Lengend Snippet: Effect of the prenatal VPA exposure on astrocytes in adulthood. Evaluation of the neurotrophin S100B and the cytoskeletal GFAP in the PfC, Cb, and HPC of healthy- (open bars, Veh) and autistic-like (black bars, VPA) adult rats (PND 90). Representation of the relative concentration of S100B ( a ) and GFAP ( c ) in VPA animals compared to control (Veh), normalized to both TBP and HPRT (ΔΔCq; N = 3, in triplicate). Representative western blots for S100B ( b ) and GFAP ( d ) proteins, and densitometric analyses normalized to β-actin used as loading control. Results are expressed as percentage of control (Veh) ( N = 3, in triplicate). Representative fluorescence micrographs of GFAP (green) staining in the PfC, GL and ML of Cb, and stratum radiatum of CA1, CA2, CA3, and the hilus of the DG of the HPC. Nuclei were stained with Hoechst (blue) ( e ). The images have been analyzed by counting the number of GFAP-positive cells in 2.4 × 10 5 μm 3 (scale bar 50 μm; N = 3, 4 times) ( f ). All data are presented as means ± SEM. Statistical analysis was performed by t test (* p < 0.05; *** p < 0.001 vs Veh group)

Article Snippet: Rabbit α-S100B , Genetex , 1:1000 , HRP conjugated goat anti-rabbit IgG 1:10000 , Jackson ImmunoResearch.

Techniques: Concentration Assay, Control, Western Blot, Fluorescence, Staining